Author(s) :

Dr. Gajanan A. Vaishnav, Dr. S. G. Vaishnav.

Volume/Issue :

Abstract :

Brain slices are thin pieces of brain tissue that are used to study the central nervous system. They are commonly prepared from mice, rats, or guinea pigs and offer several advantages over in vivo methods, including precise control over experimental conditions, the ability to examine metabolic parameters and electrophysiological properties without the influence of anaesthetics or other substances, and improved stability for electrophysiological recordings. Brain slices can be used to study a variety of brain regions and are used in research applications such as the study of neuronal membrane properties, synaptic activity, and simple circuit function. However, brain slices also have limitations, such as a lack of certain inputs and outputs present in the intact brain, damage to the slice caused by the slicing process, a limited lifespan, and the potential influence of ischemia resulting from decapitation. The protocol for preparing a rat brain slice includes using materials such as a rat brain, ice-cold cutting solution, a vibratome or slicing apparatus, microscope slides, a dissecting microscope, a scalpel or razor blade, forceps or tweezers, and cover slips

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